logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0011 : IkB-[alpha], NFkB inhibitor [alpha]

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein NamesNF-kappa-B inhibitor alpha [Homo sapiens]; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; IkappaBalpha; major histocompatibility complex enhancer-binding protein MAD3; ikB-alpha; I-kappa-B-alpha; IkB-alpha; Major histocompatibility complex enhancer-binding protein MAD3
Gene NamesNFKBIA; IKBA, MAD3, NFKBI; IKBA; MAD3; NFKBI; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Gene Locus14q13; chromosome 14
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_065390 NM_020529 4792 P25963 164008 HGNC:7797 01235 hsa:4792

* Information From OMIM

Description: NFKB1 (OMIM:164011) or NFKB2 (OMIM:164012) is bound to REL (OMIM:164910), RELA (OMIM:164014), or RELB (OMIM:604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, OMIM:604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, OMIM:600664, or IKBKB, OMIM:603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

» More (1)

* Structure Information

1. Primary Information

Length: 317 aa

Average Mass: 35.609 kDa

Monoisotopic Mass: 35.587 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 50 ---
Ankyrin repeats (many copies) 1. 728147.00.0
Ankyrin repeats (many copies) 2. 10713130.00.0
Ankyrin repeats (3 copies) 1. 13521220.00.3
Ankyrin repeats (3 copies) 2. 21624655.00.1

3. Sequence Information

Fasta Sequence: SB0011.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1IKN (X-ray; 230 A; D=67-302), 1NFI (X-ray; 270 A; E/F=70-282)

* Cleavage Information

1 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 15202778] Schaecher K, Goust JM, Banik NL, The effects of calpain inhibition on IkB alpha degradation after activation of PBMCs: identification of the calpain cleavage sites. Neurochem Res. 2004 Jul;29(7):1443-51.

Cleavage sites (±10aa)


Gln50 Glu

iTraq-117 Signal 2255.1 () for EYEQMVKELQEIR

iTraq-117 Signal 3623.7 () for EIRLEPQEVP

iTraq-117 Signal 1737.0 () for LEPQEVP

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24457201] Stanborough T, Niederhauser J, Koch B, Bergler H, Pertschy B, Ribosomal protein S3 interacts with the NF-kappaB inhibitor IkappaBalpha. FEBS Lett. 2014 Mar 3;588(5):659-64. doi: 10.1016/j.febslet.2013.12.034. Epub

» More (25)