logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0014 : ATG5-like

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesautophagy protein 5 isoform a [Homo sapiens]; autophagy protein 5 isoform a; apoptosis-specific protein; ATG5 autophagy related 5 homolog; Autophagy protein 5; APG5-like; Apoptosis-specific protein
Gene NamesATG5; APG5L, ASP; APG5L; ASP; autophagy related 5
Gene Locus6q21; chromosome 6
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_004840 NM_004849 9474 Q9H1Y0 604261 HGNC:589 16051 hsa:9474

* Information From OMIM

Function: Apoptosis is an active form of cell death and is of fundamental importance in tissue development and homeostasis. Grand et al. (1995) observed that human and rodent cells undergoing apoptosis expressed high levels of a novel 45-kD protein, which they termed apoptosis-specific protein (ASP). They also found ASP in Burkitt lymphoma (OMIM:113970) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of stimuli. The authors did not detect ASP in viable cells or in cells dying passively by necrosis. Microscopy showed high levels of ASP in the cytoplasm of cells displaying the chromatin condensation and fragmentation patterns typical of apoptosis. The authors observed retention of ASP even when DNA was no longer detectable. Immunofluorescence staining indicated that ASP primarily colocalizes with, but is clearly distinct from, nonmuscle actin (e.g., OMIM:102560). Grand et al. (1995) concluded that ASP forms part of, or at least strongly associates with, a modified cytoskeleton unique to cells undergoing apoptosis.

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* Structure Information

1. Primary Information

Length: 275 aa

Average Mass: 32.447 kDa

Monoisotopic Mass: 32.426 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Autophagy protein Apg5 1. 792701.00.2
--- cleavage 193 (inside Autophagy protein Apg5 79..270) ---

3. Sequence Information

Fasta Sequence: SB0014.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 4GDK (X-ray; 270 A; B/E=1-275), 4GDL (X-ray; 288 A; B=1-275), 4NAW (X-ray; 220 A; B/F/J/N=1-275), 4TQ0 (X-ray; 270 A; A/C/E=1-275), 4TQ1 (X-ray; 180 A; A=1-275)

* Cleavage Information

1 [sites] cleaved by Calpain 1 and/or 2

Source Reference: [PubMed ID: 16998475] Yousefi S, Perozzo R, Schmid I, Ziemiecki A, Schaffner T, Scapozza L, Brunner T, Simon HU, Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006 Oct;8(10):1124-32. Epub 2006 Sep 24.

Cleavage sites (±10aa)


Thr193 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24269628] Aporta A, Catalan E, Galan-Malo P, Ramirez-Labrada A, Perez M, Azaceta G, Palomera L, Naval J, Marzo I, Pardo J, Anel A, Granulysin induces apoptotic cell death and cleavage of the autophagy regulator Atg5 in human hematological tumors. Biochem Pharmacol. 2014 Feb 1;87(3):410-23. doi: 10.1016/j.bcp.2013.11.004. Epub

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