logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0022 : Cortactin

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namessrc substrate cortactin isoform a [Homo sapiens]; src substrate cortactin isoform a; oncogene EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); 1110020L01Rik; amplaxin; Src substrate cortactin; Amplaxin; Oncogene EMS1
Gene NamesCTTN; EMS1; cortactin
Gene Locus11q13; chromosome 11
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_005222 NM_005231 2017 Q14247 164765 HGNC:3338 01268 hsa:2017

* Information From OMIM

Description: Cortactin is a multidomain protein that functions as a key regulator of the actin cytoskeleton. It has roles in many actin-based cellular processes, including cell migration and invasion, tumor cell metastasis, and endocytosis (summary by Tegtmeyer et al., 2011).

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* Structure Information

1. Primary Information

Length: 550 aa

Average Mass: 61.585 kDa

Monoisotopic Mass: 61.549 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
TMEM210 family 1. 7910350.00.0
TMEM210 family 2. 12014054.00.1
TMEM210 family 3. 14717544.00.0
TMEM210 family 4. 18521445.00.0
TMEM210 family 5. 22924652.00.0
TMEM210 family 6. 26428550.00.0
TMEM210 family 7. 29832247.00.0
--- cleavage 336 ---
Protein of unknown function (DUF1682) 1. 342399251.015.6
--- cleavage 346 (inside Protein of unknown function (DUF1682) 342..399) ---
--- cleavage 351 (inside Protein of unknown function (DUF1682) 342..399) ---
--- cleavage 358 (inside Protein of unknown function (DUF1682) 342..399) ---
Variant SH3 domain 1. 4975472.00.0

3. Sequence Information

Fasta Sequence: SB0022.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1X69 (NMR; -; A=485-550), 2D1X (X-ray; 190 A; A/B/C/D=490-550)

* Cleavage Information

4 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 16280362] Perrin BJ, Amann KJ, Huttenlocher A, Proteolysis of cortactin by calpain regulates membrane protrusion during cell migration. Mol Biol Cell. 2006 Jan;17(1):239-50. Epub 2005 Nov 9.

Cleavage sites (±10aa)


Lys336 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Lys346 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Arg351 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Ala358 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24469447] Han SP, Gambin Y, Gomez GA, Verma S, Giles N, Michael M, Wu SK, Guo Z, Johnston W, Sierecki E, Parton RG, Alexandrov K, Yap AS, Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens. J Biol Chem. 2014 Mar 14;289(11):7764-75. doi: 10.1074/jbc.M113.544478. Epub 2014

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