logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0038 : RhoA, ras homolog gene family, member A

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namestransforming protein RhoA precursor [Homo sapiens]; transforming protein RhoA precursor; transforming protein RhoA; oncogene RHO H12; Aplysia ras-related homolog 12; small GTP binding protein RhoA; h12; rho cDNA clone 12; ras homolog gene family, member A; Transforming protein RhoA; Rho cDNA clone 12
Gene NamesRHOA; ARH12, ARHA, RHO12; ARH12; ARHA; RHO12; ras homolog family member A
Gene Locus3p21.3; chromosome 3
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001655 NM_001664 387 P61586 165390 HGNC:667 01323 hsa:387

* Information From OMIM

Function: The small guanosine triphosphatase (GTP) Rho regulates remodeling of the actin cytoskeleton during cell morphogenesis and motility. In their Figure 3C, Maekawa et al. (1999) diagrammed proposed signaling pathways for Rho-induced remodeling of the actin cytoskeleton. They demonstrated that active Rho signals to its downstream effector ROCK1 (OMIM:601702), which phosphorylates and activates LIM kinase (see OMIM:601329). LIM kinase, in turn, phosphorylates cofilin (OMIM:601442), inhibiting its actin-depolymerizing activity.

* Structure Information

1. Primary Information

Length: 193 aa

Average Mass: 21.768 kDa

Monoisotopic Mass: 21.754 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Signal recognition particle receptor beta subunit 1. 110121113.00.0

3. Sequence Information

Fasta Sequence: SB0038.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1A2B (X-ray; 240 A; A=1-181), 1CC0 (X-ray; 500 A; A/C=1-190), 1CXZ (X-ray; 220 A; A=1-181), 1DPF (X-ray; 200 A; A=1-180), 1FTN (X-ray; 210 A; A=1-193), 1KMQ (X-ray; 155 A; A=4-181), 1LB1 (X-ray; 281 A; B/D/F/H=1-190), 1OW3 (X-ray; 180 A; B=1-193), 1S1C (X-ray; 260 A; A/B=1-181), 1TX4 (X-ray; 165 A; B=3-179), 1X86 (X-ray; 322 A; B/D/F/H=1-193), 1XCG (X-ray; 250 A; B/F=3-180), 2RGN (X-ray; 350 A; C/F=1-193), 3KZ1 (X-ray; 270 A; E/F=1-181), 3LW8 (X-ray; 185 A; A/B/C/D=2-181), 3LWN (X-ray; 228 A; A/B=2-181), 3LXR (X-ray; 168 A; A=2-181), 3MSX (X-ray; 165 A; A=1-180), 3T06 (X-ray; 284 A; B/F=3-180), 4D0N (X-ray; 210 A; A=1-184), 4XH9 (X-ray; 200 A; B/E=2-180)

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 11964413] Kulkarni S, Goll DE, Fox JE, Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J Biol Chem. 2002 Jul 5;277(27):24435-41. Epub 2002 Apr 18.

Cleavage sites (±10aa)


Gln180 Ala

iTraq-117 Signal 154.8 () for FEMATRAALQAR

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24584070] Yoo HY, Sung MK, Lee SH, Kim S, Lee H, Park S, Kim SC, Lee B, Rho K, Lee JE, Cho KH, Kim W, Ju H, Kim J, Kim SJ, Kim WS, Lee S, Ko YH, A recurrent inactivating mutation in RHOA GTPase in angioimmunoblastic T cell lymphoma. Nat Genet. 2014 Apr;46(4):371-5. doi: 10.1038/ng.2916. Epub 2014 Mar 2.

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