logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0042 : Caspase 9

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namescaspase-9 isoform alpha precursor [Homo sapiens]; caspase-9 isoform alpha precursor; apoptotic protease MCH-6; apoptotic protease activating factor 3; ICE-like apoptotic protease 6; protein phosphatase 1, regulatory subunit 56; Caspase-9; CASP-9;; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10
Gene NamesCASP9; MCH6; caspase 9, apoptosis-related cysteine peptidase
Gene Locus1p36.21; chromosome 1
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001220 NM_001229 842 P55211 602234 HGNC:1511 03756 hsa:842

* Information From OMIM

Function: Li et al. (1997) determined that caspase-9 and APAF1 (OMIM:602233) bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c (OMIM:123970) and dATP, an event that leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3. Depletion of caspase-9 from S-100 extracts diminished caspase-3 activation. Mutation of the active site of caspase-9 (cys287 to ala) attenuated the activation of caspase-3 and cellular apoptotic response in vivo, indicating that caspase-9 is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP.

* Structure Information

1. Primary Information

Length: 416 aa

Average Mass: 46.280 kDa

Monoisotopic Mass: 46.251 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Caspase recruitment domain 1. 7902.00.4
--- cleavage 115 ---
Anti-Sigma Factor A 1. 16719731.00.0
--- cleavage 330 ---
Caspase recruitment domain 2. 3804049.00.0

3. Sequence Information

Fasta Sequence: SB0042.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1JXQ (X-ray; 280 A; A/B/C/D=140-416), 1NW9 (X-ray; 240 A; B=140-416), 2AR9 (X-ray; 280 A; A/B/C/D=139-416), 3D9T (X-ray; 150 A; C/D=316-321), 3V3K (X-ray; 349 A; A/C/E/G/I/K/M/O=141-416), 3YGS (X-ray; 250 A; P=1-95), 4RHW (X-ray; 210 A; E/F=1-100)

* Cleavage Information

2 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 10671558] Chua BT, Guo K, Li P, Direct cleavage by the calcium-activated protease calpain can lead to inactivation of caspases. J Biol Chem. 2000 Feb 18;275(7):5131-5.

Cleavage sites (±10aa)


Ile115 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Asp330 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24424093] Sharoar MG, Islam MI, Shahnawaz M, Shin SY, Park IS, Amyloid beta binds procaspase-9 to inhibit assembly of Apaf-1 apoptosome and intrinsic apoptosis pathway. Biochim Biophys Acta. 2014 Apr;1843(4):685-93. doi: 10.1016/j.bbamcr.2014.01.008.

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