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SB0063 : Integrin [beta]1 isoform 1A

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesintegrin beta-1 isoform 1A precursor [Homo sapiens]; integrin beta-1 isoform 1A precursor; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; glycoprotein IIa; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta
Gene NamesITGB1; FNRB, MDF2, MSK12; FNRB; MDF2; MSK12; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Gene Locus10p11.2; chromosome 10
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_002202 NM_002211 3688 P05556 135630 HGNC:6153 00628 hsa:3688

* Information From OMIM

Function: Arregui et al. (2000) demonstrated that cell-permeable (Trojan) peptides containing the third helix of the antennapedia homeodomain fused to a peptide mimicking the juxtamembrane (JMP) region of the cytoplasmic domain of N-cadherin (CDH2; OMIM:114020) result in the inhibition of both CDH2 and ITGB1 function. Microscopic analysis showed that expression of JMP, which binds to the cytoplasmic domain of CDH2, results in a reduction of neurite outgrowth on cadherin substrates. Treatment of cells with JMP resulted in the release of FER (OMIM:176942) from the cadherin complex and its accumulation in the integrin complex. The accumulation of FER in the integrin complex and the inhibitory effects of JMP could be reversed with a peptide that mimics the first coiled-coil domain of FER. The results suggested that FER mediates crosstalk between CDH2 and ITGB1.

* Structure Information

1. Primary Information

Length: 798 aa

Average Mass: 88.414 kDa

Monoisotopic Mass: 88.357 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Integrin beta tail domain 1. 33355.02.2
EGF-like domain 1. 363912.01.7
Integrin beta tail domain 2. 20722148.00.0
Integrin beta tail domain 3. 41344225.01.4
EGF-like domain 2. 4765007.018.7
EGF-like domain 3. 5165531.013.5
Integrin beta tail domain 4. 57959813.014.8
EGF-like domain 4. 5996301.016.5
EGF-like domain 5. 63864112.05.8
Integrin beta cytoplasmic domain 1. 7527951.00.4
--- cleavage 771 (inside Integrin beta cytoplasmic domain 752..795) ---
--- cleavage 777 (inside Integrin beta cytoplasmic domain 752..795) ---
--- cleavage 778 (inside Integrin beta cytoplasmic domain 752..795) ---
--- cleavage 789 (inside Integrin beta cytoplasmic domain 752..795) ---
--- cleavage 791 (inside Integrin beta cytoplasmic domain 752..795) ---

3. Sequence Information

Fasta Sequence: SB0063.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1K11 (Model; -; B=786-797), 1LHA (Model; -; A=86-543), 3G9W (X-ray; 216 A; C/D=752-785), 3T9K (X-ray; 230 A; A/B=758-769), 3VI3 (X-ray; 290 A; B/D=21-465), 3VI4 (X-ray; 290 A; B/D=21-465), 4DX9 (X-ray; 300 A; 6/7/8/9/B/D/F/H/J/L/N/P/R/T/V/X/Z/b/d/f/h/j/l/n/p/r/t/v/x/z=784-798), 4WJK (X-ray; 185 A; B=21-465), 4WK0 (X-ray; 178 A; B=21-465), 4WK2 (X-ray; 250 A; B=21-465), 4WK4 (X-ray; 250 A; B=21-465)

* Cleavage Information

5 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 10571053] Pfaff M, Du X, Ginsberg MH, Calpain cleavage of integrin beta cytoplasmic domains. FEBS Lett. 1999 Oct 22;460(1):17-22.

Cleavage sites (±10aa)


Met771 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Thr777 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Gly778 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Thr789 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Val791 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24510996] Lee C, Lee C, Lee S, Siu A, Ramos DM, The cytoplasmic extension of the integrin beta6 subunit regulates epithelial-to-mesenchymal transition. Anticancer Res. 2014 Feb;34(2):659-64.

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