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SB0070 : IGFBP4, Insulin-like growth factor binding protein 4

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesinsulin-like growth factor-binding protein 4 precursor [Homo sapiens]; insulin-like growth factor-binding protein 4 precursor; insulin-like growth factor-binding protein 4; IGF-binding protein 4; IBP-4; Insulin-like growth factor-binding protein 4; IGFBP-4
Gene NamesIGFBP4; IBP4; insulin-like growth factor binding protein 4
Gene Locus17q21.2; chromosome 17
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001543 NM_001552 3487 P22692 146733 HGNC:5473 N/A hsa:3487

* Information From OMIM

Description: Insulin-like growth factor (see IGF1; OMIM:147440)-binding proteins (IGFBPs), such as IGFBP4, are involved in the systemic and local regulation of IGF activity. IGFBPs contain 3 structurally distinct domains each comprising approximately one-third of the molecule. The N-terminal domain 1 and the C-terminal domain 3 of IGFBPs show moderate to high levels of sequence identity, including 12 and 6 invariant cysteines in domains 1 and 3, respectively (IGFBP6 (OMIM:146735) contains 10 cysteines in domain 1), and are thought to be the IGF-binding domains. Domain 2 is defined primarily by a lack of sequence identity among the IGFBPs and by a lack of cysteines, though it does contain 2 cysteines in IGFBP4. Domain 3 is homologous to the thyroglobulin type I repeat unit (Kiefer et al., 1992).

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* Structure Information

1. Primary Information

Length: 258 aa

Average Mass: 27.934 kDa

Monoisotopic Mass: 27.915 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 23 ---
Thyroglobulin type-1 repeat 1. 687522.00.1
--- cleavage 107 ---
Insulin-like growth factor binding protein 1. 13013836.00.1
--- cleavage 143 ---
--- cleavage 159 ---
Insulin-like growth factor binding protein 2. 21222811.01.2

3. Sequence Information

Fasta Sequence: SB0070.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1WQJ (X-ray; 160 A; B=24-103), 2DSP (X-ray; 250 A; B=22-113), 2DSQ (X-ray; 280 A; A/B=22-113), 2DSR (X-ray; 210 A; B=24-103, G=172-253)

* Cleavage Information

4 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 15843151] Ghosh M, Shanker S, Siwanowicz I, Mann K, Machleidt W, Holak TA, Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain. Biol Chem. 2005 Jan;386(1):85-93.

Cleavage sites (±10aa)


Glu23 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Glu107 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Phe143 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Asn159 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 23975422] Zhang Z, Yang M, Chen R, Su W, Li P, Chen S, Chen Z, Chen A, Li S, Hu C, IBP regulates epithelial-to-mesenchymal transition and the motility of breast cancer cells via Rac1, RhoA and Cdc42 signaling pathways. Oncogene. 2014 Jun 26;33(26):3374-82. doi: 10.1038/onc.2013.337. Epub 2013 Aug

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