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SB0084 : BID, BH3 interacting domain death agonist

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein NamesBH3-interacting domain death agonist isoform 2 [Homo sapiens]; BH3-interacting domain death agonist isoform 2; BH3-interacting domain death agonist; apoptic death agonist; Human BID coding sequence; desmocollin type 4; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID
Gene NamesBID; BH3 interacting domain death agonist
Gene Locus22q11.1; chromosome 22
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001187 NM_001196 637 P55957 601997 HGNC:1050 03590 hsa:637

* Information From OMIM

Function: Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8 (CASP8; OMIM:601763), the apical caspase activated by cell surface death receptors such as FAS (OMIM:134637) and TNF (OMIM:191160). Peptide mass fingerprinting identified this protein as BID. CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Immunodepletion of BID from cell extracts eliminated the cytochrome c releasing activity. The cytochrome c releasing activity of BID was antagonized by BCL2. A mutation at the BH3 domain diminished its cytochrome c releasing activity. BID, therefore, relays an apoptotic signal from the cell surface to mitochondria.

* Structure Information

1. Primary Information

Length: 195 aa

Average Mass: 21.994 kDa

Monoisotopic Mass: 21.981 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Apoptosis regulator proteins, Bcl-2 family 1. 55946.00.0
--- cleavage 70 (inside Apoptosis regulator proteins, Bcl-2 family 55..94) ---
CRM1 C terminal 1. 13118573.00.0

3. Sequence Information

Fasta Sequence: SB0084.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1ZY3 (NMR; -; B=82-101), 2BID (NMR; -; A=1-195), 2KBW (NMR; -; B=76-106), 2M5B (NMR; -; B=80-101), 2M5I (NMR; -; A=61-195), 4BD2 (X-ray; 221 A; C=76-109), 4ZEQ (X-ray; 180 A; B=79-104), 5AJJ (X-ray; 175 A; B=79-112)

* Cleavage Information

1 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 11940658] Mandic A, Viktorsson K, Strandberg L, Heiden T, Hansson J, Linder S, Shoshan MC, Calpain-mediated Bid cleavage and calpain-independent Bak modulation: two separate pathways in cisplatin-induced apoptosis. Mol Cell Biol. 2002 May;22(9):3003-13.

Cleavage sites (±10aa)


Gly70 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24158446] Wang Y, Tjandra N, Structural insights of tBid, the caspase-8-activated Bid, and its BH3 domain. J Biol Chem. 2013 Dec 13;288(50):35840-51. doi: 10.1074/jbc.M113.503680. Epub

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