logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0101 : C-terminal binding protein 1

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein NamesC-terminal-binding protein 1 isoform 1 [Homo sapiens]; C-terminal-binding protein 1 isoform 1; brefeldin A-ribosylated substrate; C-terminal-binding protein 1; CtBP1; 1.1.1.-
Gene NamesCTBP1; CTBP; C-terminal binding protein 1
Gene Locus4p16; chromosome 4
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001319 NM_001328 1487 Q13363 602618 HGNC:2494 04015 hsa:1487

* Information From OMIM

Function: Polycomb (Pc) is part of a Pc group (PcG) protein complex that is involved in repression of gene activity during Drosophila and vertebrate development. Using a yeast 2-hybrid assay, Sewalt et al. (1999) found that Xenopus Ctbp1 interacts with Xenopus Pc and that human CTBP2 interacts with PC2 (OMIM:603079), a human Pc homolog. Immunofluorescence studies indicated that CTBP1 and CTBP2 partially colocalize with PC2 in large PcG domains in interphase nuclei. As with PC2, chimeric LexA-CTBP2 and LexA-CTBP1 proteins repressed gene activity when targeted to a reporter gene. Sewalt et al. (1999) suggested that PC2-mediated repression of gene expression involves an association with corepressors such as the CTBPs. They speculated that the interference of the adenoviral E1A protein with the transcription machinery of the infected cell may involve interference with PcG-mediated repression through disruption of the CTBP-PcG interaction. Northern blot analysis revealed that the CTBP1 gene was expressed as a 2.4-kb mRNA in all human tissues tested.

* Structure Information

1. Primary Information

Length: 440 aa

Average Mass: 47.535 kDa

Monoisotopic Mass: 47.505 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Cellulose synthase subunit D 1. 17019888.00.0
XdhC Rossmann domain 1. 33737444.00.0

3. Sequence Information

Fasta Sequence: SB0101.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1MX3 (X-ray; 195 A; A=28-353), 4LCE (X-ray; 238 A; A=28-353), 4U6Q (X-ray; 230 A; A=28-353), 4U6S (X-ray; 210 A; A=28-353)

* Cleavage Information

2 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 23707407] Ono Y, Iemura S, Novak SM, Doi N, Kitamura F, Natsume T, Gregorio CC, Sorimachi H, PLEIAD/SIMC1/C5orf25, a novel autolysis regulator for a skeletal-muscle-specific calpain, CAPN3, scaffolds a CAPN3 substrate, CTBP1. J Mol Biol. 2013 Aug 23;425(16):2955-72. doi: 10.1016/j.jmb.2013.05.009. Epub

Cleavage sites (±10aa)


Asn375 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Gly387 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 23907728] Bizama C, Benavente F, Salvatierra E, Gutierrez-Moraga A, Espinoza JA, Fernandez EA, Roa I, Mazzolini G, Sagredo EA, Gidekel M, Podhajcer OL, The low-abundance transcriptome reveals novel biomarkers, specific intracellular pathways and targetable genes associated with advanced gastric cancer. Int J Cancer. 2014 Feb 15;134(4):755-64. doi: 10.1002/ijc.28405. Epub 2013 Aug

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