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SB0109 : PDLIM1

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein NamesPDZ and LIM domain protein 1 [Homo sapiens]; PDZ and LIM domain protein 1; carboxyl terminal LIM domain protein 1; epididymis secretory protein Li 112; elfin; LIM domain protein CLP-36; C-terminal LIM domain protein 1; Elfin
Gene NamesPDLIM1; CLIM1, CLP36; CLIM1; CLP36; PDZ and LIM domain 1
Gene Locus10q23.1; chromosome 10
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_066272 NM_020992 9124 O00151 605900 HGNC:2067 09326 hsa:9124

* Information From OMIM

Function: By yeast 2-hybrid analysis, Kotaka et al. (2000) showed that the LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2; OMIM:102573). Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL; OMIM:193065), which is localized in the fascia adherens of intercalated discs.

* Structure Information

1. Primary Information

Length: 329 aa

Average Mass: 36.071 kDa

Monoisotopic Mass: 36.049 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
PDZ domain 1. 288516.00.0
Domain of unknown function (DUF4749) 1. 1382331.01.9
Restriction alleviation protein Lar 1. 2573093.02.5
--- cleavage 271 (inside Restriction alleviation protein Lar 257..309) ---

3. Sequence Information

Fasta Sequence: SB0109.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1X62 (NMR; -; A=250-315), 2PKT (X-ray; 150 A; A=1-86)

* Cleavage Information

1 [sites] cleaved by Calpain 3

Source Reference: [PubMed ID: 19926129] Bertipaglia I, Bourg N, Richard I, Pahlman AK, Andersson L, James P, Carafoli E, A proteomic study of calpain-3 and its involvement in limb girdle muscular dystrophy type 2a. Cell Calcium. 2009 Nov-Dec;46(5-6):356-63. doi: 10.1016/j.ceca.2009.10.003. Epub

Cleavage sites (±10aa)


Phe271 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 22922308] Sikorska M, Krezel A, Otlewski J, Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability. J Inorg Biochem. 2012 Oct;115:28-35. doi: 10.1016/j.jinorgbio.2012.05.009. Epub

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