logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0118 : Rad21

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesdouble-strand-break repair protein rad21 homolog [Homo sapiens]; double-strand-break repair protein rad21 homolog; protein involved in DNA double-strand break repair; nuclear matrix protein 1; NXP-1; SCC1 homolog; sister chromatid cohesion 1; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1
Gene NamesRAD21; HR21, KIAA0078, NXP1; HR21; KIAA0078; NXP1; RAD21 homolog (S. pombe)
Gene Locus8q24; chromosome 8
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_006256 NM_006265 5885 O60216 606462 HGNC:9811 05924 hsa:5885

* Information From OMIM

Description: Eukaryotic sister chromatids remain connected from the time of synthesis until they are separated in anaphase. This cohesion depends on a complex of proteins known as cohesins. In vertebrates, unlike in yeast, the cohesins dissociate from chromosome arms earlier in M phase, during prophase. Small amounts of cohesin remain near the centromere until metaphase, with complete removal at the beginning of anaphase. Cohesin complexes contain SCC1 (RAD21), SMC1 (OMIM:300040), SMC3 (CSPG6; OMIM:606062), and either SA1 (STAG1; OMIM:604358) or SA2 (STAG2; OMIM:300826). The complexes, in turn, interact with PDS5 (see OMIM:613200), a protein implicated in chromosome cohesion, condensation, and recombination in yeast (summary by Sumara et al., 2000).

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* Structure Information

1. Primary Information

Length: 631 aa

Average Mass: 71.689 kDa

Monoisotopic Mass: 71.645 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
Conserved region of Rad21 / Rec8 like protein 1. 11223.00.0
--- cleavage 192 ---
DNA polymerase phi 1. 526577646.06.4

3. Sequence Information

Fasta Sequence: SB0118.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 4PJU (X-ray; 305 A; B=281-420), 4PJW (X-ray; 285 A; B=281-420), 4PK7 (X-ray; 295 A; B=281-420)

* Cleavage Information

1 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 21876002] Panigrahi AK, Zhang N, Mao Q, Pati D, Calpain-1 cleaves Rad21 to promote sister chromatid separation. Mol Cell Biol. 2011 Nov;31(21):4335-47. doi: 10.1128/MCB.06075-11. Epub 2011 Aug

Cleavage sites (±10aa)


Leu192 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24148822] Mahmood SF, Gruel N, Chapeaublanc E, Lescure A, Jones T, Reyal F, Vincent-Salomon A, Raynal V, Pierron G, Perez F, Camonis J, Del Nery E, Delattre O, Radvanyi F, Bernard-Pierrot I, A siRNA screen identifies RAD21, EIF3H, CHRAC1 and TANC2 as driver genes within the 8q23, 8q24.3 and 17q23 amplicons in breast cancer with effects on cell growth, survival and transformation. Carcinogenesis. 2014 Mar;35(3):670-82. doi: 10.1093/carcin/bgt351. Epub 2013 Oct

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