logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0129 : CAPN2, m-calpain catalytic subunit, mCL

[ CaMP Format ]

* Basic Information

OrganismRattus norvegicus (Norway rat)
Protein Namescalpain-2 catalytic subunit precursor [Rattus norvegicus]; calpain-2 catalytic subunit precursor; calpain-2 catalytic subunit; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calcium-activated neutral proteinase 2; CANP 2; Calpain-2 catalytic subunit; 3.4.22.53; Calcium-activated neutral proteinase 2; Calpain M-type; Calpain-2 large subunit; Millimolar-calpain
Gene NamesCapn2; calpain 2, (m/II) large subunit
Gene Locus13q26; chromosome 13
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_058812 NM_017116 29154 Q07009 N/A N/A N/A rno:29154

* Information From OMIM

Not Available.

* Structure Information

1. Primary Information

Length: 700 aa

Average Mass: 79.918 kDa

Monoisotopic Mass: 79.869 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 1 ---
--- cleavage 2 ---
--- cleavage 3 ---
--- cleavage 7 ---
--- cleavage 9 ---
--- cleavage 10 ---
--- cleavage 11 ---
--- cleavage 12 ---
--- cleavage 13 ---
--- cleavage 14 ---
--- cleavage 23 ---
Calpain family cysteine protease 1. 463421.00.0
--- cleavage 60 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 82 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 116 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 201 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 207 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 209 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 247 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 249 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 255 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 267 (inside Calpain family cysteine protease 46..342) ---
--- cleavage 359 ---
--- cleavage 360 ---
--- cleavage 381 ---
--- cleavage 383 ---
--- cleavage 387 ---
--- cleavage 390 ---
--- cleavage 392 ---
--- cleavage 407 ---
--- cleavage 409 ---
--- cleavage 410 ---
--- cleavage 423 ---
CorA-like Mg2+ transporter protein 1. 43952767.00.1
--- cleavage 449 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 461 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 462 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 468 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 469 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 502 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 509 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 513 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 517 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 524 (inside CorA-like Mg2+ transporter protein 439..527) ---
--- cleavage 525 (inside CorA-like Mg2+ transporter protein 439..527) ---
EF-hand domain pair 1. 55259820.00.1
EF-hand domain pair 2. 61062131.00.0
--- cleavage 611 (inside EF-hand domain pair 610..621) ---
--- cleavage 618 (inside EF-hand domain pair 610..621) ---
EF hand 1. 65766817.00.0
EF hand 2. 6756804.00.0

3. Sequence Information

Fasta Sequence: SB0129.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1DF0 (X-ray; 260 A; A=1-700), 1MDW (X-ray; 195 A; A/B=19-346), 1QXP (X-ray; 280 A; A/B=1-700), 1U5I (X-ray; 286 A; A=1-700), 3BOW (X-ray; 240 A; A=1-700), 3DF0 (X-ray; 295 A; A=1-700)

* Cleavage Information

45 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 21549862] Chou JS, Impens F, Gevaert K, Davies PL, m-Calpain activation in vitro does not require autolysis or subunit dissociation. Biochim Biophys Acta. 2011 Jul;1814(7):864-72. doi: 10.1016/j.bbapap.2011.04.007.

Cleavage sites (±10aa)

[Site 1] M1-AGIAMKLAKD

Met1 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
---------Met1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala2Gly3Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11

Sequence conservation (by blast)

[Site 2] MA2-GIAMKLAKDR

Ala2 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
--------Met1Ala2
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly3Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11Arg12

Sequence conservation (by blast)

[Site 3] MAG3-IAMKLAKDRE

Gly3 Ile

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
-------Met1Ala2Gly3
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11Arg12Glu13

Sequence conservation (by blast)

[Site 4] MAGIAMK7-LAKDREAAEG

Lys7 Leu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
---Met1Ala2Gly3Ile4Ala5Met6Lys7
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Leu8Ala9Lys10Asp11Arg12Glu13Ala14Ala15Glu16Gly17

Sequence conservation (by blast)

[Site 5] MAGIAMKLA9-KDREAAEGLG

Ala9 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
-Met1Ala2Gly3Ile4Ala5Met6Lys7Leu8Ala9
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys10Asp11Arg12Glu13Ala14Ala15Glu16Gly17Leu18Gly19

Sequence conservation (by blast)

[Site 6] MAGIAMKLAK10-DREAAEGLGS

Lys10 Asp

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Met1Ala2Gly3Ile4Ala5Met6Lys7Leu8Ala9Lys10
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asp11Arg12Glu13Ala14Ala15Glu16Gly17Leu18Gly19Ser20

Sequence conservation (by blast)

[Site 7] AGIAMKLAKD11-REAAEGLGSH

Asp11 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala2Gly3Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Arg12Glu13Ala14Ala15Glu16Gly17Leu18Gly19Ser20His21

Sequence conservation (by blast)

[Site 8] GIAMKLAKDR12-EAAEGLGSHE

Arg12 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gly3Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11Arg12
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu13Ala14Ala15Glu16Gly17Leu18Gly19Ser20His21Glu22

Sequence conservation (by blast)

[Site 9] IAMKLAKDRE13-AAEGLGSHER

Glu13 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ile4Ala5Met6Lys7Leu8Ala9Lys10Asp11Arg12Glu13
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala14Ala15Glu16Gly17Leu18Gly19Ser20His21Glu22Arg23

Sequence conservation (by blast)

[Site 10] AMKLAKDREA14-AEGLGSHERA

Ala14 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala5Met6Lys7Leu8Ala9Lys10Asp11Arg12Glu13Ala14
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala15Glu16Gly17Leu18Gly19Ser20His21Glu22Arg23Ala24

Sequence conservation (by blast)

[Site 11] AAEGLGSHER23-AIKYLNQDYE

Arg23 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ala14Ala15Glu16Gly17Leu18Gly19Ser20His21Glu22Arg23
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala24Ile25Lys26Tyr27Leu28Asn29Gln30Asp31Tyr32Glu33

Sequence conservation (by blast)

[Site 12] FPALPSSLGF60-KELGPYSSKT

Phe60 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Phe51Pro52Ala53Leu54Pro55Ser56Ser57Leu58Gly59Phe60
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys61Glu62Leu63Gly64Pro65Tyr66Ser67Ser68Lys69Thr70

Sequence conservation (by blast)

[Site 13] IEWKRPTEIC82-ADPQFIIGGA

Cys82 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ile73Glu74Trp75Lys76Arg77Pro78Thr79Glu80Ile81Cys82
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala83Asp84Pro85Gln86Phe87Ile88Ile89Gly90Gly91Ala92

Sequence conservation (by blast)

[Site 14] LLAAIASLTL116-NEEILARVVP

Leu116 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Leu107Leu108Ala109Ala110Ile111Ala112Ser113Leu114Thr115Leu116
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Asn117Glu118Glu119Ile120Leu121Ala122Arg123Val124Val125Pro126

Sequence conservation (by blast)

[Site 15] YEALSGGATT201-EGFEDFTGGI

Thr201 Glu

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Tyr192Glu193Ala194Leu195Ser196Gly197Gly198Ala199Thr200Thr201
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Glu202Gly203Phe204Glu205Asp206Phe207Thr208Gly209Gly210Ile211

Sequence conservation (by blast)

[Site 16] GATTEGFEDF207-TGGIAEWYEL

Phe207 Thr

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Gly198Ala199Thr200Thr201Glu202Gly203Phe204Glu205Asp206Phe207
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Thr208Gly209Gly210Ile211Ala212Glu213Trp214Tyr215Glu216Leu217

Sequence conservation (by blast)

[Site 17] TTEGFEDFTG209-GIAEWYELRK

Gly209 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Thr200Thr201Glu202Gly203Phe204Glu205Asp206Phe207Thr208Gly209
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly210Ile211Ala212Glu213Trp214Tyr215Glu216Leu217Arg218Lys219

Sequence conservation (by blast)

[Site 18] LGCSIDITSA247-ADSEAVTYQK

Ala247 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Leu238Gly239Cys240Ser241Ile242Asp243Ile244Thr245Ser246Ala247
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ala248Asp249Ser250Glu251Ala252Val253Thr254Tyr255Gln256Lys257

Sequence conservation (by blast)

[Site 19] CSIDITSAAD249-SEAVTYQKLV

Asp249 Ser

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Cys240Ser241Ile242Asp243Ile244Thr245Ser246Ala247Ala248Asp249
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Ser250Glu251Ala252Val253Thr254Tyr255Gln256Lys257Leu258Val259

Sequence conservation (by blast)

[Site 20] SAADSEAVTY255-QKLVKGHAYS

Tyr255 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Ser246Ala247Ala248Asp249Ser250Glu251Ala252Val253Thr254Tyr255
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gln256Lys257Leu258Val259Lys260Gly261His262Ala263Tyr264Ser265

Sequence conservation (by blast)

[Site 21] LVKGHAYSVT267-GAEEVESSGS

Thr267 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Leu258Val259Lys260Gly261His262Ala263Tyr264Ser265Val266Thr267
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Gly268Ala269Glu270Glu271Val272Glu273Ser274Ser275Gly276Ser277

Sequence conservation (by blast)

[Site 22] CDSYKKWKLT359-KMDGNWRRGS

Thr359 Lys

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
Cys350Asp351Ser352Tyr353Lys354Lys355Trp356Lys357Leu358Thr359
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'
Lys360Met361Asp362Gly363Asn364Trp365Arg366Arg367Gly368Ser369

Sequence conservation (by blast)