logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0138 : Antiapoptotic Bfl-1 (BCL2A1)

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesbcl-2-related protein A1 isoform 2 [Homo sapiens]; bcl-2-related protein A1 isoform 2; hematopoietic BCL2-related protein A1; bcl2-L-5; protein BFL-1; bcl-2-like protein 5; hemopoietic-specific early response protein; Bcl-2-related protein A1; Bcl-2-like protein 5; Bcl2-L-5; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS
Gene NamesBCL2A1; BCL2L5, BFL1, GRS, HBPA1; BCL2L5; BFL1; GRS; HBPA1; BCL2-related protein A1
Gene Locus15q24.3; chromosome 15
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_001108207 NM_001114735 597 Q16548 601056 HGNC:991 N/A hsa:597

* Information From OMIM

Function: D'Sa-Eipper et al. (1996) showed that the BFL1 protein suppresses apoptosis induced by the p53 tumor suppressor protein in a manner similar to other BCL2 family members. The BFL1 gene showed a dominant cooperating oncogenic activity with the E1A oncogene in transformation of primary rodent epithelial cells.

* Structure Information

1. Primary Information

Length: 163 aa

Average Mass: 18.745 kDa

Monoisotopic Mass: 18.734 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 21 ---
--- cleavage 22 ---
DDE_Tnp_1-associated 1. 468646.00.0
--- cleavage 71 (inside DDE_Tnp_1-associated 46..86) ---
DDE_Tnp_1-associated 2. 11612439.00.0

3. Sequence Information

Fasta Sequence: SB0138.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 2VM6 (X-ray; 220 A; A=1-149), 3I1H (X-ray; 220 A; A=1-151), 3MQP (X-ray; 224 A; A=1-151), 4ZEQ (X-ray; 180 A; A=1-151)

* Cleavage Information

3 [sites] cleaved by Calpain 1

Source Reference: [PubMed ID: 22745672] Valero JG, Cornut-Thibaut A, Juge R, Debaud AL, Gimenez D, Gillet G, Bonnefoy-Berard N, Salgado J, Salles G, Aouacheria A, Kucharczak J, micro-Calpain conversion of antiapoptotic Bfl-1 (BCL2A1) into a prodeath factor reveals two distinct alpha-helices inducing mitochondria-mediated apoptosis. PLoS One. 2012;7(6):e38620. doi: 10.1371/journal.pone.0038620. Epub 2012 Jun 20.

Cleavage sites (±10aa)


Leu21 Gln

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Gln22 Ile

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Phe71 Asn

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24810636] Medina-Echeverz J, Haile LA, Zhao F, Gamrekelashvili J, Ma C, Metais JY, Dunbar CE, Kapoor V, Manns MP, Korangy F, Greten TF, IFN-gamma regulates survival and function of tumor-induced CD11b+ Gr-1high myeloid derived suppressor cells by modulating the anti-apoptotic molecule Bcl2a1. Eur J Immunol. 2014 Aug;44(8):2457-67. doi: 10.1002/eji.201444497. Epub 2014 Jun

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