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SB0142 : [alpha]-actinin-4

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namesalpha-actinin-4 [Homo sapiens]; alpha-actinin-4; focal segmental glomerulosclerosis 1; actinin alpha4 isoform; non-muscle alpha-actinin 4; F-actin cross-linking protein; Alpha-actinin-4 {ECO:0000305}; Non-muscle alpha-actinin 4 {ECO:0000303|PubMed:10656685}
Gene NamesACTN4; ACTN4 {ECO:0000312|HGNC:HGNC:166}; actinin, alpha 4
Gene Locus19q13; chromosome 19
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_004915 NM_004924 81 O43707 604638 HGNC:166 05222 hsa:81

* Information From OMIM

Function: Using immunohistochemistry, El-Husseini et al. (2000) showed that rat RNF22 and ACTN4 are expressed in a punctate pattern throughout the cytoplasm and neuritic processes of differentiated PC12 cells. They concluded that the isoform of ACTN4 they identified may act to anchor microfilaments to various cellular structures.

* Structure Information

1. Primary Information

Length: 911 aa

Average Mass: 104.853 kDa

Monoisotopic Mass: 104.788 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 13 ---
Phage integrase SAM-like domain 1. 506777.00.0
CAMSAP CH domain 1. 698911.00.0
CH-like domain in sperm protein 1. 18326512.00.0
Up-regulated During Septation 1. 26930778.00.0
--- cleavage 283 (inside Up-regulated During Septation 269..307) ---
Up-regulated During Septation 2. 4325286.03.0
Phage integrase SAM-like domain 2. 62169449.00.3
Phage integrase SAM-like domain 3. 75077845.00.1
EF-hand domain pair 1. 80883625.00.0
EF hand 1. 86186816.00.0

3. Sequence Information

Fasta Sequence: SB0142.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1WLX (NMR; -; A=519-645), 1YDI (X-ray; 180 A; B=734-757), 2R0O (X-ray; 220 A; A/B=47-271)

* Cleavage Information

2 [sites] cleaved by Calpain 2

Source Reference: [PubMed ID: 23466492] Shao H, Travers T, Camacho CJ, Wells A, The carboxyl tail of alpha-actinin-4 regulates its susceptibility to m-calpain and thus functions in cell migration and spreading. Int J Biochem Cell Biol. 2013 Jun;45(6):1051-63. doi:

Cleavage sites (±10aa)


Tyr13 Gly

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Lys283 Val

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 23899839] Noro R, Honda K, Tsuta K, Ishii G, Maeshima AM, Miura N, Furuta K, Shibata T, Tsuda H, Ochiai A, Sakuma T, Nishijima N, Gemma A, Asamura H, Nagai K, Yamada T, Distinct outcome of stage I lung adenocarcinoma with ACTN4 cell motility gene amplification. Ann Oncol. 2013 Oct;24(10):2594-600. doi: 10.1093/annonc/mdt293. Epub 2013 Jul

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