logo CaMPDB: Calpain for Modulatory Proteolysis Database

SB0150 : CAPN3, p94, calpain-3

[ CaMP Format ]

* Basic Information

OrganismHomo sapiens (human)
Protein Namescalpain-3 isoform a [Homo sapiens]; calpain-3 isoform a; calpain, large polypeptide L3; calpain p94, large [catalytic] subunit; muscle-specific calcium-activated neutral protease 3 large subunit; new calpain 1; Calpain-3;; Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3; New calpain 1; nCL-1
Gene NamesCAPN3; CANP3, CANPL3, NCL1; CANP3; CANPL3; NCL1; calpain 3, (p94)
Gene Locus15q15.1; chromosome 15
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_000061 NM_000070 825 P20807 114240 HGNC:1480 00255 hsa:825

* Information From OMIM

Description: The CAPN3 gene encodes calpain-3. The calpains, or calcium-activated neutral proteases (EC, are nonlysosomal intracellular cysteine proteases. Mammalian calpains are heterodimers composed of a ubiquitous 80-kD large subunit (e.g., CAPN1, OMIM:114220 and CAPN2, OMIM:114230) and a common small 30-kD subunit (CAPNS1; OMIM:114170). CAPN3 is a muscle-specific large subunit (Sorimachi et al., 1989).

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* Structure Information

1. Primary Information

Length: 821 aa

Average Mass: 94.253 kDa

Monoisotopic Mass: 94.194 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
--- cleavage 14 ---
--- cleavage 30 ---
--- cleavage 33 ---
EF-hand domain pair 1. 39440911.00.0
--- cleavage 412 ---
Calpain large subunit, domain III 1. 4295781.00.0
Unstructured region on Calpain-3 1. 5836531.010.8
EF-hand domain pair 2. 66872016.00.1
EF-hand domain pair 3. 73074431.00.0
EF-hand domain pair 4. 79680932.00.5

3. Sequence Information

Fasta Sequence: SB0150.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1Y9V (Model; -; A/B=649-821), 4OKH (X-ray; 245 A; A/B/C=642-821)

* Cleavage Information

4 [sites] cleaved by Calpain 3

Source Reference: [PubMed ID: 14594950] Ono Y, Kakinuma K, Torii F, Irie A, Nakagawa K, Labeit S, Abe K, Suzuki K, Sorimachi H, Possible regulation of the conventional calpain system by skeletal muscle-specific calpain, p94/calpain 3. J Biol Chem. 2004 Jan 23;279(4):2761-71. Epub 2003 Nov 1.

Cleavage sites (±10aa)


Ala14 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Lys30 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Glu33 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Glu412 Ile

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 24126726] Ruffini F, Tentori L, Dorio AS, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal PM, Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness. Oncol Rep. 2013 Dec;30(6):2887-96. doi: 10.3892/or.2013.2791. Epub 2013 Oct 10.

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