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XSB0121 : caspase 9 isoform beta preproprotein [Homo sapiens]

[ CaMP Format ]

This entry is computationally expanded from SB0042

* Basic Information

OrganismHomo sapiens (human)
Protein NamesCaspase-9; CASP-9;; ICE-like apoptotic protease 6; ICE-LAP6; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; Caspase-9 subunit p35; Caspase-9 subunit p10; caspase 9 isoform beta preproprotein; caspase 9; apoptosis-related cysteine protease; apoptotic protease activating factor 3
Gene NamesCASP9; MCH6; caspase 9, apoptosis-related cysteine peptidase
Gene Locus1p36.3-p36.1; chromosome 1
GO FunctionNot available
Entrez Protein Entrez Nucleotide Entrez Gene UniProt OMIM HGNC HPRD KEGG
NP_127463 NM_032996 842 P55211 602234 1511 N/A hsa:842

* Information From OMIM

Function: Li et al. (1997) determined that caspase-9 and APAF1 (OMIM:602233) bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c (OMIM:123970) and dATP, an event that leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3. Depletion of caspase-9 from S-100 extracts diminished caspase-3 activation. Mutation of the active site of caspase-9 (cys287 to ala) attenuated the activation of caspase-3 and cellular apoptotic response in vivo, indicating that caspase-9 is the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP.

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* Structure Information

1. Primary Information

Length: 266 aa

Average Mass: 30.184 kDa

Monoisotopic Mass: 30.165 kDa

2. Domain Information

Annotated Domains: interpro / pfam / smart / prosite

Computationally Assigned Domains (Pfam+HMMER):

domain namebeginendscoree-value
CARD 1. 69291.53e-24

3. Sequence Information

Fasta Sequence: XSB0121.fasta

Amino Acid Sequence and Secondary Structures (PsiPred):

4. 3D Information

Known Structures in PDB: 1JXQ (X-ray; 280 A; A/B/C/D=140-416), 1NW9 (X-ray; 240 A; B=140-416), 2AR9 (X-ray; 280 A; A/B/C/D=140-416), 3D9T (X-ray; 150 A; C/D=316-321), 3YGS (X-ray; 250 A; P=1-95)

* Cleavage Information

2 [sites]

Cleavage sites (±10aa)


Asp180 Ala

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)


Ile115 Arg

P10 P9 P8 P7 P6 P5 P4 P3 P2 P1
P1' P2' P3' P4' P5' P6' P7' P8' P9' P10'

Sequence conservation (by blast)

* References

[PubMed ID: 19269008] Kim YR, Kim KM, Yoo NJ, Lee SH, Mutational analysis of CASP1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 14 genes in gastrointestinal stromal tumors. Hum Pathol. 2009 Jun;40(6):868-71. Epub 2009 Mar 9.

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